易志刚

简介：副研究员， 博士研究生导师， PI, 复旦大学基础医学院病原生物系教育部／卫计委医学分子病毒学重点实验室 PI, 上海市重大传染病及生物安全研究院 副主任，科学研究部，复旦大学公共卫生临床中心 教育背景 1996-2000 学士，青岛海洋大学 2000-2003 硕士，中国海洋大学（原青岛海洋大学） 2003-2006 博士，复旦大学 工作经历 2021.1-今 双聘PI，上海市重大传染病及生物安全研究院 2020.2-今 双聘教授，副主任，科学研究部，复旦大学上海市公共卫生临床中心 2017.9-2020.2 兼职教授，副主任，应急检测及生物安全部，复旦大学上海市公共卫生临床中心 2014.1-今 副研究员，复旦大学基础医学院病原生物系分子病毒学实验室 2009.3-2012.6 博士后，洛克菲勒大学 2006.7-2013.12 讲师，复旦大学基础医学院病原生物系分子病毒学实验室 主要研究方向和专长： 研究方向：研究RNA病毒，包括黄热病病毒、丙型肝炎病毒、肠道病毒及流感病毒等的复制机理及致病机制；病原体鉴定。 论文发表（*通讯作者）： 47. Ma X, Yuan Z, Yi Z*. Identification and characterization of key residues in Zika virus envelope protein for virus assembly and entry. Emerg Microbes Infect. 2022 Dec;11(1):1604-1620 46. Yi Z, Yuan Z. From discovery to cure, a great journey of the hepatitis C virus study. Infect Dis Immun. 2022;2(2):109–112. 45. Song W, Yang Y, Huang Y, Chen L, Shen Z, Yuan Z*, Fu L*, Yi Z*. Acute respiratory infections in children, before and after the COVID-19 pandemic, a sentinel study. J Infect. 2022 Apr 5:S0163-4453(22)00183-9 44. Jiang H, Yang T, Yang C, Lu Y, Yi Z, Zhang Q, Wang W. Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020. Arch Virol. 2022 Apr;167(4):1111-1123. 43. Qian X, Wu B, Tang H, Luo Z, Xu Z, Ouyang S, Li X, Xie J, Yi Z, Leng Q, Liu Y, Qi Z, Zhao P. Rifapentine is an entry and replication inhibitor against yellow fever virus both in vitro and in vivo. Emerg Microbes Infect. 2022 Dec;11(1):873-884 42. Wang LP, Yuan Y, Liu YL, Lu QB, Shi LS, Ren X, Zhou SX, Zhang HY, Zhang XA, Wang X, Wang YF, Lin SH, Zhang CH, Geng MJ, Li J, Zhao SW, Yi ZG, Chen X, Yang ZS, Meng L, Wang XH, Cui AL, Lai SJ, Liu MY, Zhu YL, Xu WB, Chen Y, Yuan ZH, Li MF, Huang LY, Jing HQ, Li ZJ, Liu W, Fang LQ, Wu JG, Hay SI, Yang WZ, Gao GF; Chinese Centers for Disease Control and Prevention (CDC) Etiology of Acute Meningitis and Encephalitis Surveillance Study Team. Etiological and epidemiological features of acute meningitis or encephalitis in China: a nationwide active surveillance study. Lancet Reg Health West Pac. 2022 Jan 3;20:100361 41. Peng H, Ding C, Jiang L, Tang W, Liu Y, Zhao L, Yi Z, Ren H, Li C, He Y, Zheng X, Tang H, Chen Z, Qi Z, Zhao P. Discovery of potential anti-SARS-CoV-2 drugs based on large-scale screening in vitro and effect evaluation in vivo. Sci China Life Sci. 2021 Dec 24:1-17. 40. Ren L, Zhang W, Zhang J, Zhang J, Zhang H, Zhu Y, Meng X, Yi Z*, Wang R*. Influenza A Virus (H1N1) Infection Induces Glycolysis to Facilitate Viral Replication. Virol Sin. 2021 Dec;36(6):1532-1542 39. Li ZJ, Zhang HY, Ren LL, Lu QB, Ren X, Zhang CH, Wang YF, Lin SH, Zhang XA, Li J, Zhao SW, Yi ZG, Chen X, Yang ZS, Meng L, Wang XH, Liu YL, Wang X, Cui AL, Lai SJ, Jiang T, Yuan Y, Shi LS, Liu MY, Zhu YL, Zhang AR, Zhang ZJ, Yang Y, Ward MP, Feng LZ, Jing HQ, Huang LY, Xu WB, Chen Y, Wu JG, Yuan ZH, Li MF, Wang Y, Wang LP, Fang LQ, Liu W, Hay SI, Gao GF, Yang WZ; Chinese Centers for Disease Control and Prevention (CDC) Etiology of Respiratory Infection Surveillance Study Team. Etiological and epidemiological features of acute respiratory infections in China. Nat Commun. 2021 Aug 18;12(1):5026 38. Su J, Ma X, Zhang Y, Zou J, Yuan Z*, Yi Z*. NS5-independent ablation of STAT2 by Zika virus to antagonize interferon signaling. Emerg Microbes Infect. 2021 Dec;10(1):1609-1625 37. Zhang J, Zhang Y, Kang JY, Chen S, He Y, Han B, Liu MF, Lu L, Li L, Yi Z*, Chen L*. Potential transmission chains of variant B.1.1.7 and co-mutations of SARS-CoV-2. Cell Discov. 2021 Jun 15;7(1):44 36. Wang LP, Zhou SX, Wang X, Lu QB, Shi LS, Ren X, Zhang HY, Wang YF, Lin SH, Zhang CH, Geng MJ, Zhang XA, Li J, Zhao SW, Yi ZG, Chen X, Yang ZS, Meng L, Wang XH, Liu YL, Cui AL, Lai SJ, Liu MY, Zhu YL, Xu WB, Chen Y, Wu JG, Yuan ZH, Li MF, Huang LY, Li ZJ, Liu W, Fang LQ, Jing HQ, Hay SI, Gao GF, Yang WZ; Chinese Centers for Disease Control and Prevention (CDC) Etiology of Diarrhea Surveillance Study Team. Etiological, epidemiological, and clinical features of acute diarrhea in China. Nat Commun. 2021 Apr 29;12(1):2464 35. Zhang Y, Chen, S, Yuan Z*, Yi Z*. Bioorthogonal dissection of the replicase assembly of hepatitis C virus. Cell Chemical Biology. 2021 September 16, 28:1366–1378 (featured article) 34. Jia X, Hu L, Wu M, Ling Y, Wang W, Lu H, Yuan Z, Yi Z*, Zhang X*.A streamlined clinical metagenomic sequencing protocol for rapid pathogen identification. Sci Rep. 2021 Feb 23;11(1):4405 33. Zhang Y, Song W, Chen, S, Yuan Z*, Yi, Z*. A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2. Antiviral Res. 2021 Jan;185:104974 32. Song W#, Jia X#, Zhang X*, Ling Y*, Yi Z*. Co-infection in COVID-19, a cohort study. J Infect. 2021 Mar;82(3):440-442 31. Yi Z#*, Ling Y#, Zhang X#, Chen J#, Hu K, Wang Y, Song W, Ying T, Zhang R, Lu H*, Yuan Z*. Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population. Emerg Microbes Infect. 2020 Dec;9(1):1988-1996 (#, co-first authors) 30. Mei X, Zhang Y, Zhu H, Ling Y, Zou Y, Zhang Z, Guo H, Liu Y, Cheng X, Liu M, Huang W, Wang J, Yi Z*, Qian Z*, Lu H*. Observations about Symptomatic and Asymptomatic infections of 494 patients with COVID-19 in Shanghai, China. Am J Infect Control. 2020:S0196-6553(20)30646-5. 29. Zhang X#, Tan Y#, Ling Y#, Lu G#, Liu F#, Yi Z#, Jia X, Wu M, Shi B, Xu S, Chen J, Wang W, Chen B, Jiang L, Yu S, Lu J, Wang J, Xu M, Yuan Z, Zhang Q, Zhang X, Zhao G, Wang S*, Chen S*, Lu H*. Viral and Host Factors Related to the Clinical Outcome of COVID-19. Nature. 2020, 583(7816): 437-440 (#, co-first authors) 28. Yan J, Wang M, Wang M, Dun Y, Zhu L, Yi Z*, Zhang S*. Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species. Virus Res. 2020 Jul 2;283:197974 27. Wang M, Yan J, Zhu L, Wang M, Liu L, Yu R, Chen M, Xun J, Zhang Y, Yi Z, Zhang S. The Establishment of Infectious Clone and Single Round Infectious Particles for Coxsackievirus A10. Virol Sin. 2020 Aug;35(4):426-435 26. 张荣#，易志刚#，王玉燕#，滕峥#，徐巍，宋武慧，蔡霞，孙志平，谷陈建，周艳秋，陈洪友，叶荣，韩文东，朱云凯，冯飞，房方皓，李崇山，张曦，瞿涤，付晨，谢幼华，袁正宏。新型冠状病毒上海株（nCoV-SH01）的分离和鉴定。微生物与感染，2020, 15 (1): 15-20 (#, 共同第一作者) 25. Mei X, Dai F, Zou J, Zhang H, Yuan Z*, Qian Z*, Yi Z*. Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance. J Med Virol. 2020, 1-7 24. Zhang H, Song Z, Zou J, Feng Y, Zhang J, Ren L, Zhang X, Hu Y, Yuan Z*, Yi Z*. An infectious clone of enterovirus 71(EV71) that is capable of infecting neonatal immune competent mice without adaptive mutations. Emerg Microbes Infect. 2020, 9(1):427-438. 23. 宋武慧，潘滨，阚海东，徐燕意*，易志刚*。安全、便捷技术再生一次性医用口罩的实验研究。微生物与感染，2020, 15 (1): 31-35 22. 宋武慧，易志刚*。丙型肝炎病毒高水平复制细胞株的构建及其机制初步研究。微生物与感染，2020, 15 (5): 285-292 21. Zhang Y, Zhao X, Zou J, Yuan Z*, Yi Z*. Dual role of the amphipathic helix of hepatitis C virus NS5A in the viral polyprotein cleavage and replicase assembly. Virology. 2019; 535: 283-296 20. Xiang C, Du Y, Meng G, Soon Yi L, Sun S, Song N, Zhang X, Xiao Y, Wang J, Yi Z, Liu Y, Xie B, Wu M, Shu J, Sun D, Jia J, Liang Z, Sun D, Huang Y, Shi Y, Xu J, Lu F, Li C, Xiang K, Yuan Z, Lu S, Deng H. Long-term functional maintenance of primary human hepatocytes in vitro. Science. 2019; 364(6438):399-402 19. Ren L, Zhang W, Han P, Zhang J, Zhu Y, Meng X, Zhang J, Hu Y, Yi Z, Wang R. Influenza A virus (H1N1) triggers a hypoxic response by stabilizing hypoxia-inducible factor-1α via inhibition of proteasome. Virology. 2019; 530:51-58. 18. Zhang Y, Zou J, Zhao, X, Yuan Z*, Yi, Z*. Hepatitis C virus NS5A inhibitor daclatasvir allosterically impairs NS4B-involved protein-protein interactions within the viral replicase and disrupts the replicase quaternary structure in a replicase assembly surrogate system. J Gen Virol. 2019;100(1):69-83 17. Du X, Zhang Y, Zou J, Yuan Z*, Yi, Z*. Replicase-mediated shielding of the poliovirus replicative double-stranded RNA to avoid recognition by MDA5. J Gen Virol. 2018;99(9):1199-1209 16. Zhang H, Song Z, Yu H, Zhang X, Xu S, Li Z, Li J, Xu H, Yuan Z, Ma H, Yi Z*, Hu Y. Genome-wide linear B-cell epitopes of enterovirus 71 in a hand, foot and mouth disease (HFMD) population. J Clin Virol. 2018;105:41-48 15. 许军，杜晓婷，张杨，袁正宏*，易志刚*。丙型肝炎病毒感染过程中其NS3/4A蛋白酶切割线粒体抗病毒信号蛋白的动态过程。微生物与感染， 2017, 12(5):270-278 14. Yi Z*, Yuan Z*. Hepatitis C Virus-Associated Cancers. Adv Exp Med Biol, 2017, 1018:129-146. Review paper. 13. Yi Z*, Yuan Z*. Aggregation of a Hepatitis C Virus Replicase Module Induced by Ablation of p97/VCP. J Gen Virol. 2017 Jul;98(7):1667-1678. 12. Du X, Pan T, Xu J, Zhang Y, Song W, Yi Z*, Yuan Z*. Hepatitis C virus replicative double-stranded RNA is a potent interferon inducer that triggers interferon production through MDA5. J Gen Virol. 2016 Nov;97(11):2868-2882. 11. Yi Z#*, Fang C#, Zou, J, Xu J, Song W, Du X, Pan T, Lu H, Yuan Z*. Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator. J Virol. 2016, 90(21): 9953-9966. (#, co-first authors) 10. Bozzacco L, Yi Z, Andreo U, Conklin CR, Li MM, Rice CM, MacDonald MR. Chaperone-Assisted Protein Folding Is Critical for Yellow Fever Virus NS3/4A Cleavage and Replication. J Virol. 2016, 90(6):3212-28 (spotlight article selected by editors) 9. Yi Z, Chen J, Kozlowski M, Yuan Z. Innate detection of hepatitis B and C virus and viral inhibition of the response. Cell Microbiol. 2015, 17(9):1295-1303. Review paper. 8. Yi, Z, Yuan, Z, Rice CM, Macdonald MR. Flavivirus replication complex assembly revealed by DNAJC14 functional mapping. J Virol. 2012, 86(21): 11815-11832. (selected by Faculty of 1000, microbiology) 7. Yi Z#, Pan T#, Wu X, Song W, Wang S, Xu Y, Rice CM, Macdonald MR, Yuan Z. Hepatitis C virus co-opts Ras-GTPase-activating protein-binding protein 1 for its genome replication. J Virol. 2011; 85(14): 6996-7004 (#, co-first authors) (spotlight article selected by editors). 6. Yi Z, Sperzel L, Nürnberger C, Bredenbeek PJ, Lubick KJ, Best SM, Stoyanov CT, Law LM, Yuan Z, Rice CM, MacDonald MR. Identification and characterization of the host protein DNAJC14 as a broadly active flavivirus replication modulator. PLoS Pathog. 2011;7(1):e1001255. 5. Yi Z#, Fang C#, Pan T, Wang J, Yang P, Yuan Z. Subproteomic study of hepatitis C virus replicon reveals Ras-GTPase-activating protein binding protein 1 as potential HCV RC component. Biochem Biophys Res Commun. 2006; 350:174-178 (#, co-first authors) 4. Fang C#, Yi Z#, Liu F, Lan S, Wang J, Lu H, Yang P, Yuan Z. Proteome analysis of human liver carcinoma Huh7 cells harboring hepatitis C virus subgenomic replicon. Proteomics. 2006; 6:519-27(#, co-first authors) 3. Pan T#, Fang C#, Yi Z#, Yang P, Yuan Z. Subproteomic analysis of the cellular proteins associated with the 3' untranslated region of the hepatitis C virus genome in human liver cells. Biochem Biophys Res Commun. 2006; 347:683-91(#, co-first authors) 2. Wang J, Tong W, Zhang X, Chen L, Yi Z, Pan T, Hu Y, Xiang L, Yuan Z. Hepatitis C virus non-structural protein NS5A interacts with FKBP38 and inhibits apoptosis in Huh7 hepatoma cells. FEBS Lett. 2006; 580:4392-400. 1. Lin S, Wu M, Xu Y, Xiong W, Yi Z, Zhang X, Yuan Z. Inhibition of hepatitis B virus replication by MyD88 is mediated by nuclear factor-kappaB activation. Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease. 2007; 1772:1150-1157 课题承担情况： 已结题课题： 1.国家自然科学基金青年基金 项目起止年限：2008-2010 项目名称： G3BP1调控 HCV 复制的研究 资助经额：17万 2.国家重点基础研究发展计划（973计划）子课题 项目起止年限：2009－2013 项目名称： HCV 复制的分子细胞机制 资助经额：165万 3.复旦大学“卓学计划” 项目起止年限：2013－2016 资助经额：15万 4. 国家重点基础研究发展计划（973计划）子课题 项目起止年限：2015－2019 项目名称：中国主要流行株HCV的病毒学特征和抗病毒治疗的应答规律研究 （2015CB554301） 资助经额：139万 在研课题： 1. 国家科技重大专项课题 项目起止年限：2017－2020 项目名称：上海地区重点RNA病毒的变异变迁规律研究 （2017ZX10103009-004） 资助经额：244万 2. 国家自然科学基金面上项目 项目起止年限：2018－2021 项目名称：宿主AAA+ATPase p97/VCP作为复制复合体重要组成参与单正链RNA病毒复制共同机制的研究 （81772181） 资助经额：53万 3. 国家自然科学基金面上项目 项目起止年限：2020－2023 项目名称：丙型肝炎病毒NS5A两性alpha-螺旋调控病毒复制复合体组装的机制研 究 （81971926） 资助经额：54万 4.上海市科委 项目起止年限：2020－2021 项目名称：上海市新型冠状病毒感染的肺炎流行病学研究 （20411950100） 资助经额：80万 专利申请： 国内发明专利： 1. 寨卡病毒MR766毒株的感染性克隆及其应用，易志刚, 袁正宏。2018101322778 (2018,2,9) 2. 基于EV71毒株的感染性cDNA克隆及其应用, 易志刚，宋志刚，张慧英，袁正宏。2019104740883 （2019,6,2） 3. 病毒SARS-CoV-2的特异性线性优势表位及对应多肽及其制药用途，易志刚, 袁正宏。202010665490.2（2020,7,11） 4. 基于细菌人工染色体的新冠病毒SARS-CoV-2亚基因组复制子克隆、构建及应用, 易志刚, 袁正宏。202010940386.X (2020,9.9) 参加学术活动情况： 2021,10。2021上海传染病论坛暨申江健康论坛-新冠肺炎的研究进展，上海。邀请报告：Toward understanding the replication complex assembly of SARS-CoV-2, lessons from Hepatitis C virus。 2021,10。2021复旦新药创制论坛，上海。邀请报告：Targeting the ACE of RNA viruses for antiviral development。 2021,4。 中华医学会新发传染病研究与预防研讨会，广东佛山。邀请报告：复制酶复合物组装机制－从HCV到SARS-CoV-2。 2019, October, 26rd International Symposium on Hepatitis C Virus and Related Viruses, Seoul, South Korea. Poster presentation: Dual role of the amphipathic helix of hepatitis C virus NS5A in the viral polyprotein cleavage and replicase assembly. 2019, July, Fudan-UoM/Doherty Infection and Immunity workshop. Doherty Institute, University of Melbourne, Melbourne, Australia. Oral presentation: Dissecting the replicase assembly of hepatitis C virus 2018, October, VIth ICGEB Workshop on Human RNA Viruses, Shanghai, China. Oral presentation（口头报告）: Mechanism of action of a direct-anting antiviral (DAA) targeting a non-enzymatic protein of Hepatitis C Virus (HCV). 2017, March, Targeting the viral replication complex assembly for effective antiviral strategy. Invited lecture. Shanghai Public Health Clinical Center. Shanghai, China 2016, October, 23rd International Symposium on Hepatitis C Virus and Related Viruses, Kyoto, Japan. Oral presentation（口头报告）: Affinity purification of the Hepatitis C virus replicase identifies Valosin-containing protein (VCP) as an active viral replication modulator. Poster presentation（板报交流）: Hepatitis C virus replicative double-stranded RNA is a potent interferon inducer that triggers interferon production through MDA5. 2016, 1。 全国丙型肝炎病毒研讨会。广东广州。口头报告: Viral and cellular determinants of innate sensing of HCV. 2015, October. 22th International Symposium on Hepatitis C Virus and Related Viruses. Strasbourg, France. Poster presentation（板报交流）: Identification and characterization of host factors associated with the HCV replicase. 2010, May. The ninth international symposium on positive-strand RNA viruses. Atlanta, USA. Poster presentation（板报交流）. 2007, October. 3rd Pasteur-Areva Course on Blood-borne virus: HCV, Institute Pasteur of Shanghai, Shanghai, China. Invited speaker （特邀报告）. 2007, September. 14th International Symposium on Hepatitis C Virus and Related Viruses. Glasgow, Scotland, UK. Oral presentation （口头报告）. 2007, May. Sino-German symposium on hepatitis, Wuhan, China. Invited speaker （特邀报告）. 学术任职： 上海市微生物学会会员 中国微生物学会会员 中国医疗保健国际交流促进会分子诊断学分会委员 教学情况： 医学微生物学理论课（本科生） 医学微生物学实验课（本科生） 医学微生物学MBBS（本科生） 医学分子病毒学II（研究生） 医学分子病毒学I（研究生） 医学分子病毒/细菌学实验（研究生） FIST 课程：肝脏生物学、免疫学及病理生理学前沿 新发传染病 （本科生） 教学获奖情况： 教材编写： 参与编写《医学微生物学》“丙型肝炎病毒”章节，复旦大学出版社，2016 参与编写《治疗性疫苗》第二版“丙型肝炎治疗性疫苗” 章节，科学出版社，2020